MIGS-1: Mixed Ginsenoside Certified Reference Material
The following table shows those constituents for which certified values have been established for this certified reference material (CRM). Certified values are based on results from data generated at NRC using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR).
The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide and k is the coverage factor. A coverage factor of two (2) was applied for all elements. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement.
|Ginesnoside (a)||CAS Number||Molecular formula||Mass fraction, mg/g|
|Rb1||41753-43-9||54H92O23||1.249 ± 0.016|
|Rb2||11021-13-9||C53H90O22||0.252 ± 0.002|
|Rb3||68406-26-8||C53H90O22||0.023 ± 0.002|
|Rc||11021-14-0||C53H90O22||0.505 ± 0.005|
|Rd||52705-93-8||C48H82O18||0.507 ± 0.006|
|Re||52286-59-6||C48H82O18||0.506 ± 0.002|
|Rg1||22427-39-0||C42H72O14||0.258 ± 0.005|
The coding refers to the instrumental method of analyte determination.
- a - quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR)
This reference material is primarily intended for use in the calibration of instrumentation, including LCUV and LC-MS, for the quantitative and/or qualitative analysis of ginsenosides. The ginsenosides are certified on a mass fraction basis in methanol.
Conversion to mass concentration (mass per volume) may be done assuming that the density of the material is that of pure methanol. Note, however, that the density of methanol changes by 0.1% per degree Celsius (in the interval from 10 to 30 °C).
The uncertainties of each integral were calculated according to the JCGM Guide and combined using the DerSimonian-Laird (DSL) variance weighting procedure. Included in the combined uncertainty estimate (uc) are uncertainties in the batch characterization and uncertainties related to possible between-bottle variation and stability. However, given that homogeneity and stability components were deemed insignificant, the combined uncertainty is identical to the characterization uncertainty. The expanded uncertainty (UCRM) in the certified values is equal to UCRM = 2uc where uc is the combined standard uncertainty.
Date of issue: April 2015
Date of expiry: April 2020
Revised: March 2016 (editorial update)
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