HYDR-1: Certified Reference Material of Hydrastine

The following tables show those constituents for which certified and information values have been established for this hydrastine purity certified reference material (CRM).

The certified value shows the purity of hydrastine. Certified values are based on results from data generated at NRC using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR) and chemical specifications. The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide and k is the coverage factor. A coverage factor of two (2) was applied for all elements. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement.

Table 1: Certified quantity value for HYDR-1

Substance Molecular formula Mass fraction,
hydrastine (a) C21H21NO6 0.993 ± 0.010

Table 2: Information values for impurities in HYDR-1

Substance Molecular formula Mass fraction,
methylene chloride (a) CH2Cl2 0.0024

All alkaloids are identified here using their common names. The Chemical Abstract Services (CAS) Registry Number of (1S,9R)-β-hydrastine is CAS 29617-43-4.


The coding refers to the instrumental method of analyte determination.

  • a – Proton nuclear magnetic resonance spectroscopy (1H-NMR)

Intended use

This reference material is primarily intended for use in the calibration of procedures and the development of methods for the determination of hydrastine. A minimum sample mass of 25 mg is recommended.


Included in the combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-bottle variation (uhom), and uncertainties related to short-term stability (ustability). Expressed as standard uncertainties, these components are listed in Table 3.

Table 3: Uncertainly Components for HYDR-1

Substance uc,
hydrastine 0.005 0.0014 0.003 0.004

Date of issue: April 2015
Date of expiry: April 2025
Revised: March 2016 (editorial update)

Date modified: