GINX-1: North American Ginseng Root Extract Certified Reference Material for Ginsenosides and Metals

The following tables show those constituents for which certified, reference and information values have been established for this North American ginseng root extract Certified Reference Material (CRM).

The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide [1] and k is the coverage factor. A coverage factor of two (2) was applied for all substances and elements. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement. All listed values are expressed on an as is basis with moisture and residual solvent data reported separately.

Table 1: Certified values for GINX-1

Ginsenoside CAS Number Molecular formula Mass fraction, mg/g
Rg1 (a,b) 22427-39-0 C42H72O14 3.28 ± 0.63
Re (a,b) 52286-59-6 C48H82O18 20.46 ± 0.89
Rb1 (a,b) 41753-43-9 C54H92O23 42.85 ± 1.21
Rc (a,b) 11021-14-0 C53H90O22 14.95 ± 1.44
Rb2 (b) Table 1 note 1 11021-13-9 C53H90O22 2.29 ± 0.72 Table 1 note 1
Rb3 (a,b) 68406-26-8 C53H90O22 3.76 ± 0.74
Rd (a,b) 52705-93-8 C48H82O18 16.55 ± 0.71

Table notes

Table 1 note 1

Rb2 is given a reference value but is listed in this Table for convenience.

Return to table 1 note 1 referrer

Table 1 (continued): Certified values for GINX-1

Element Mass fraction, mg/kg
arsenic (d,e,f,g) 0.61 ± 0.14
cadmium (c,e) 0.0145 ± 0.0010
copper (e,f) 5.43 ± 0.30
lead (c,e) 0.594 ± 0.076
magnesium (e,f,g) 1146 ± 64
manganese (e,f,g) 17.3 ± 1.6
nickel (e,f) 0.92 ± 0.20
potassium (e,f,g) 22 690 ± 500
zinc (e,f) 23.1 ± 1.4

Table 2: Reference values for GINX-1

Element Mass fraction, mg/kg
boron (e,g) 10.2 ± 3.4
calcium (e,f) 61 ± 20
chromium (e,f,g) 0.51 ± 0.22
cobalt (e,f) 0.37 ± 0.06
iron (e,f,g) 188 ± 28
molybdenum (e,f,g) 0.296 ± 0.052
mercury (c) 0.0088 ± 0.0018
phosphorus (e,f,g) 1510 ± 260
strontium (e,f) 0.79 ± 0.20

Table 3: Information values for GINX-1

Element Mass fraction, mg/kg
aluminium (e,f) 408
barium (e) 1.66
uranium (e) 0.04
vanadium (e,f) 0.36

Coding

The coding refers to the instrumental method of analyte determination.

  • a - Liquid chromatography UV spectroscopy (LC-UV)
  • b - Liquid chromatography mass spectrometry (LC-MS)
  • c - Isotope dilution inductively-coupled plasma mass spectrometry (ID-ICP-MS)
  • d - Standard addition inductively-coupled plasma mass spectrometry (SA-ICP-MS)
  • e - Inductively-coupled plasma mass spectrometry (ICP-MS)
  • f - Standard addition inductively-coupled plasma atomic emission spectroscopy (SA-ICP-AES)
  • g - Inductively-coupled plasma atomic emission spectroscopy (ICP-AES)

Intended use

This reference material is primarily intended for the development of methods for the determination of ginsenosides and major, minor, and trace constituent elements in ginseng root and other similar food, agricultural or biological materials. A minimum sample mass of 500 mg is recommended for ginsenoside determination and 250 mg for metals determination.

Storage and sampling

It is recommended that the material be stored at –20 oC. Each bottle is packaged in a trilaminate foil pouch which serves as an impermeable vapour barrier. The bottle contents should be well mixed by rotation and shaking prior to use, and tightly closed immediately thereafter. Certified values are based on a minimum 500 mg for ginsenoside sub-samples and 250 mg for metal sub-samples.

Uncertainty

Included in the overall combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-bottle variation (uhom), and uncertainties related to inconsistency between the various measurement methods (umethod). Expressed as standard uncertainties, these components are listed in Table 4.

Table 4: Uncertainty components for GINX-1

Ginsenoside uc,
mg/g
uchar,
mg/g
uhom,
mg/g
umethod,
mg/g
Rg1 0.31 0.09 0.30 0.03
Re 0.45 0.31 0.32 0.04
Rb1 0.61 0.38 0.33 0.33
Rc 0.72 0.17 0.32 0.62
Rb2 0.36 0.19 0.30 0.00
Rb3 0.37 0.07 0.30 0.21
Rd 0.35 0.16 0.30 0.09

Table 4 (continued): Uncertainty components for GINX-1

Element uc,
mg/kg
uchar,
mg/kg
uhom,
mg/kg
umethod,
mg/kg
arsenic 0.07 0.07 0.00 0.00
cadmium 0.0005 0.0005 0.00 0.00
copper 0.15 0.13 0.07 0.00
lead 0.038 0.020 0.015 0.028
magnesium 32 18 26 0
manganese 0.8 0.6 0.5 0.0
nickel 0.10 0.07 0.07 0.00
potassium 250 210 130 0
zinc 0.7 0.2 0.7 0.0

Table 4 (continued): Uncertainty components for GINX-1

Element uc,
mg/kg
uchar,
mg/kg
uhom,
mg/kg
umethod,
mg/kg
boron 1.7 1.5 0.0 0.8
calcium 10 8 0 6
chromium 0.11 0.11 0.03 0.00
cobalt 0.03 0.03 0.00 0.00
iron 14 14 0 0
molybdenum 0.026 0.026 0.000 0.000
mercury 0.0009 0.0009 0.0000 0.0000
phosphorus 130 100 0 90
strontium 0.10 0.03 0.02 0.09

Date of issue: February 2017
Date of expiry: February 2022

Date modified: