FEBS-1: Otolith certified reference material for trace metals

FEBS-1 is a saggital otolith reference material procured from red snapper (Lutjanus campechanus). Certified values are based on unweighted mean results from data submitted by collaborating laboratories. The expanded uncertainty (UCRM) in the certified value is equal to U = kuc, where uc is the combined standard uncertainty calculated according to the JCGM Guide and k is the coverage factor. The value of uc is determined from the combined uncertainties of the vairous analytical methods (uchar) as well as uncertainties associated with homogeneity (uhom).

It is intended that UCRM encompasses every aspect that reasonably contributes to the uncertainty of the measurement. A coverage factor k = 2 was applied for all elements. The table below lists those elements for which certified values have been established.

Table 1: Certified quantity values

Element Mass fraction Unit
Barium (d,i,p) 5.09 ± 0.23 mg/kg
Lithium (d,p) 0.305 ± 0.044 mg/kg
Magnesium (d,i,p) 23.6 ± 1.3 mg/kg
Manganese(d,n) 0.686 ± 0.016 mg/kg
Sodium (d,i,n) 2594 ± 161 mg/kg
Strontium (d,i,p) 2055 ± 79 mg/kg
Calcium (d,i,n,x) 38.3 ± 1.4 g/100g

Coding

The coding refers only to the instrumental method of analyte determination.

  • d - Inductively coupled plasma mass spectrometry (ICP-MS)
  • i - Inductively coupled plasma mass spectrometry
  • n - Neutron activation (INAA)
  • p - Isotope dilution inductively coupled plasma mass spectrometry (ID-ICPMS)
  • x - X-ray fluorescence spectrometry (XRF).

Intended use

This reference material is primarily intended for use in the calibration of procedures and the development of methods used for the analysis of bulk elemental composition of otoliths and other marine aragonites.

Table 2: Uncertainty components

Incertainty u (wBa), mg/kg u (wCa), g/100g u (wLi), mg/kg u (wMg), mg/kg u (wMn), mg/kg u (wNa), mg/kg u (wSr), mg/kg
uchar 0.039 0.62 0.0211 0.395 0.006 72.9 21
uhom 0.108 0.35 0.0055 0.535 0.0055 23.5 34
uc 0.115 0.717 0.022 0.665 0.0081 80.3 40
U (k=2) 0.23 1.4 0.044 1.33 0.016 161 79

Table 3: Information values

Element Interval Technique
Cd 1.4 - 3.2 μg/kg IDICPMS, ICPMS
Cu 4.2 - 6.8 mg/kg IDICPMS, ICPMS
Ni 16 - 29 μg/kg IDICPMS
Pb 0.40 - 0.77 mg/kg IDICPMS, ICPMS
Zn 3.2 - 6.3 mg/kg IDICPMS, INAA

Date of issue: August 2005
Date of expiry: April 2015
Revised: January 2015 (expiry date extended, layout and editorial changes), March 2016 (editorial update)

Date modified: