DOLT-5: Dogfish Liver Certified Reference Material for Trace Metals and other Constituents

The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide and k = 2 is the coverage factor. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement. All listed values are expressed on a dry mass basis.

Table 1: Certified Quantity Values

Element/compound Mass fraction, (mg/kg)
Arsenic (b,d) 34.6 ± 2.4
Cadmium (a,d) 14.5 ± 0.6
Calcium (c) 550 ± 80
Cobalt (b,d) 0.267 ± 0.026
Copper (a,c,d) 35.0 ± 2.4
Iron (a,c,d) 1070 ± 80
Lead (a,d) 0.162 ± 0.032
Magnesium (c) 940 ± 100
Mercury (a,e) 0.44 ± 0.18
Molybdenum (a,d) 1.41 ± 0.22
Potassium (c) 14 400 ± 3000
Selenium (a,d) 8.3 ± 1.8
Silver (a,d) 2.05 ± 0.08
Sodium (c) 9 900 ± 1600
Strontium (a,c,d) 3.73 ± 0.26
Tin (a,d) 0.069 ± 0.036
Vanadium (b,d) 0.51 ± 0.06
Zinc (a,c,d) 105.3 ± 5.4
Arsenobetaine (as As) (g,h) 24.2 ± 0.8

Table 2: Reference Values

Element/compound Mass fraction, (mg/kg)
Aluminium (c,d) 31.7 ± 4.2
Nickel (a,d) 1.71 ± 0.56
Chromium (a,d) 2.35 ± 0.58
Methylmercury (as Hg) (f) 0.119 ± 0.058
Manganese (d) 8.91 ± 0.70

Table 3: Information Values

Element Mass fraction, (mg/kg)
Antimony (d) 0.013
Phosphorus (d) 11 500
Thallium (d) 0.013
Uranium (d) 0.082

Coding

The coding refers to the instrumental method of analyte determination.

  • a - Isotope dilution inductively-coupled plasma mass spectrometry (ID‑ICP‑MS)
  • b - Standard addition inductively-coupled plasma mass spectrometry (ICP‑MS)
  • c - Inductively-coupled plasma atomic emission spectroscopy (ICP‑AES)
  • d - Inductively-coupled plasma mass spectrometry (ICP‑MS)
  • e - Cold-vapour atomic emission spectroscopy (CV‑AAS)
  • f - Isotope dilution gas chromatography ICP‑MS (ID‑GC ICP‑MS)
  • g - Standard addition liquid chromatography ICP‑MS (LC‑ICP‑MS)
  • h - Isotope dilution liquid chromatography Orbitrap mass spectrometry

Intended Use

This reference material is primarily intended for use in the calibration of procedures and the development of methods for the determination of trace and matrix constituents in marine sediments and materials with similar matrices. A minimum sample mass of 250 mg is recommended.

Uncertainty

Included in the overall combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-bottle variation (uhom), and uncertainties related to inconsistency between the various measurement methods (umethod). Expressed as standard uncertainties, these components are listed below:

Table 4: Uncertainty Components

Element/compound uc,
mg/kg
uchar,
(mg/kg)
uhom,
(mg/kg)
umethod,
(mg/kg)
Arsenic 1.2 0.8 0.9 0.0
Cadmium 0.3 0.2 0.2 0.0
Calcium 40 30 20 0
Cobalt 0.013 0.009 0.010 0.000
Copper 1.2 0.6 1.0 0.0
Iron 40 20 30 0
Lead 0.016 0.007 0.014 0.000
Magnesium 50 40 30 0
Mercury 0.09 0.02 0.05 0.07
Molybdenum 0.11 0.11 0.03 0.00
Potassium 1500 1400 400 0
Selenium 0.9 0.3 0.8 0.0
Silver 0.04 0.03 0.03 0.00
Sodium 800 700 300 0
Strontium 0.13 0.09 0.09 0.00
Tin 0.018 0.003 0.017 0.004
Vanadium 0.03 0.02 0.02 0.00
Zinc 2.7 1.9 1.9 0.0
Arsenobetaine(as As) 0.4 0.3 0.2 0.0

Table 4 (continued): Uncertainty components for DOLT-5

Element/compound uc,
mg/kg
uchar,
(mg/kg)
uhom,
(mg/kg)
umethod,
(mg/kg)
Aluminium 2.1 1.0 1.8 0.0
Nickel 0.28 0.12 0.25 0.00
Chromium 0.29 0.15 0.25 0.00
Methylmercury (as Hg) 0.029 0.011 0.009 0.025
Manganese 0.35 0.35 0.00 0.00

Date of issue: August 2014
Date of expiry: August 2019
Revised: January 2016 (editorial changes and typographical errors for U and Tl in table 3 corrected), March 2016 (editorial update), August 2017 (arsenobetaine included)

Date modified: