CARP-2: Ground whole carp reference material for organochlorine compounds

This reference material is primarily intended for use in the calibration of procedures and the development of methods used for the determination of PCB's, PCDD's, PCDF's and pesticides in biological materials.

Table 1: Certified mass fractions for selected polychlorinated biphenyl (PCB) congeners in CARP-2

Congener
(IUPAC No.)
Mass fraction, μg/kg,
(wet weight basis)
18 27.3 ± 4.0
28 34.0 ± 7.2
44 86.6 ± 25.9
52 138 ± 43
118 148 ± 33
128 20.4 ± 4.4
153 105 ± 22
180 53.3 ± 13.0
194 10.9 ± 3.1
206 4.4 ± 1.1

The certified results are expressed as the mean value ± the expanded uncertainty (Table 1). These results were derived from four contributions: (1) the NRC analysis, (2) the mean of three methods performed at NIST, (3) the recommended value arising from the NIST/NOAA intercomparison, (4) the mean from three results performed by an external laboratory.

Table 2: Reference mass fractions for selected polychlorinated biphenyl (PCB) congeners in CARP-2

Congener
(IUPAC No.)
Mass fraction, μg/kg,
(wet weight basis)
8 4.8 ± 1.8
66/95 174 ± 52
101/90 145 ± 48
105 53.2 ± 15.6
138/163/164 103 ± 30
170/190 20.6 ± 2.9
187/182 37.1 ± 6.3
209 4.6 ± 2.0

Mass fractions of selected PCBs are provided as reference values because limited data was available concerning the relative amounts of co-eluting congeners. The results are expressed as the mean value ± the expanded uncertainty (Table 2). The PCB reference results were derived from four contributions: (1) the NRC analysis, (2) the mean of three methods performed at NIST, (3) the recommended value arising from the NIST/NOAA intercomparison Footnote3 and (4) the mean of three results performed by an external laboratory.

Table 3: Reference mass fractions for selected pesticides in CARP-2

Pesticides Mass fraction, μg/kg,
(wet weight basis)
gamma-chlordane 4.5 ± 0.7
2,4'-DDE 2.9 ± 0.5
trans-nonachlor 11.0 ± 0.9
dieldrin 8.3 ± 0.8
4,4'-DDE 158 ± 14
2,4'-DDD 21.8 ± 0.7
4,4'-DDD 90.9 ± 8.5

Mass fractions of selected pesticides are provided as reference values because the variance from multiple methods was greater than desired for certified values. The results are expressed as the mean value ± the expanded uncertainty (Table 3). The pesticide reference results were derived from three contributions: (1) the NRC analysis, (2) the mean of three methods performed at NIST, (3) the recommended value arising from the NIST/NOAA intercomparison.

Table 4 lists the polychlorinated dibenzo-p-dioxin (PCDD) and polychlorinated dibenzofuran (PCDF) congeners for which reference values have been established for CARP-2.

These mass fractions are provided as reference values because limited data were available to permit certification. The results are expressed as the mean value ± the expanded uncertainty. The reference result is the mean of five independent analyses performed at NRC.

Table 4: Reference mass fractions for selected PCDF's and PCDD's in CARP-2

Congener Mass fraction, μg/kg,
(wet weight basis)
Polychlorinated dibenzofuran (PCDF)
2,3,7,8-Tetrachlorodibenzofuran 18.2 ± 1.6
1,2,3,7,8-Pentachlorodibenzofuran 5.6 ± 0.3
Polychlorinated dibenzo-p-dioxin (PCDD)
2,3,7,8-Tetrachlorodibenzo-p-dioxin 7.4 ± 0.7
1,2,3,7,8-Pentachlorodibenzo-p-dioxin 5.3 ± 1.3
1,2,3,4,7,8-Hexachlorodibenzo-p-dioxin 1.6 ± 0.3
1,2,3,6,7,8-Hexachlorodibenzo-p-dioxin 5.8 ± 0.8
1,2,3,4,7,8-Hexachlorodibenzo-p-dioxin 0.78 ± 0.12
1,2,3,4,6,7,8-Heptachlorodibenzo-p-dioxin 6.4 ± 0.9
Octachlorodibenzo-p-dioxin 9.4 ± 1.7

Date of issue: July 1, 2001
Date of expiry: July 2021
Revised: January 2013, March 2016 (editorial changes and expiry date extended)

Date modified: