CANA-1: Certified reference material of canadine

The following tables show those constituents for which certified and information values have been established for this canadine purity certified reference material (CRM).

The certified value shows the purity of canadine. Certified values are based on results from data generated at NRC using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR) and chemical specifications. The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide and k is the coverage factor. A coverage factor of two (2) was applied for all elements. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement.

Table 1: Certified quantity value for CANA‑1

Substance Element Mass fraction,
canadine (a) C20H21NO4 0.9936 ± 0.0059

Table 2: Information values for impurities in CANA‑1

Substance Molecular formula Mass fraction,
berberine chloride (b) C20H18NO4Cl 0.000 20
tetrahydropalmatine (b,c) C21H25NO4 0.000 37
tetrahydrojatrorrhizine (a,b,c) C20H23NO4 0.0024
methanol (a) CH3OH 0.0014
methylene chloride (a) CH2Cl2 0.0011

All alkaloids are identified here using their common names. The corresponding Chemical Abstract Services (CAS) Registry Numbers are as follows: canadine (tetrahydroberberine, CAS 522-97-4), berberine chloride (CAS 633-65-8), tetrahydropalmatine (CAS 2934-97-6), and tetrahydrojatrorrhizine (CAS 6018-39-9).


The coding refers to the instrumental method of analyte determination.

  • a – Proton nuclear magnetic resonance spectroscopy (1H‑NMR)
  • b – Liquid chromatography mass spectrometry (LC‑MS)
  • c – Liquid chromatography UV-Vis spectroscopy (LC‑UV)

Intended use

This reference material is primarily intended for use in the calibration of procedures and the development of methods for the determination of canadine. A minimum sample mass of 25 mg is recommended.


Included in the combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-bottle variation (uhom), and uncertainties related to short-term stability (ustability). Expressed as standard uncertainties, these components are listed in Table 3.

Table 3: Uncertainty Components for CANA‑1

Substance uc,
canadine 0.0030 0.0008 0.0016 0.0024

Date of issue: April 2015
Date of expiry: April 2025
Revised: March 2016 (editorial update)

Date modified: