ABET-1: Certified Reference Material of natural arsenobetaine bromide

The following tables show those constituents for which certified and reference values have been established for this arsenobetaine purity certified reference material (CRM).

The certified value shows the purity of arsenobetaine bromide. Certified values are based on results from data generated at NRC using quantitative proton nuclear magnetic resonance spectroscopy (1H-qNMR) and chemical specifications. The expanded uncertainty (UCRM) in the certified value is equal to U = kuc where uc is the combined standard uncertainty calculated according to the JCGM Guide [1] and k is the coverage factor. A coverage factor of two (2) was applied. It is intended that UCRM accounts for every aspect that reasonably contributes to the uncertainty of the measurement.

Table 1: Certified quantity values for ABET-1

Substance Molecular formula Mass fraction,
arsenobetaine bromide (a) C5H12AsO2Br 0.9930 ± 0.0050
arsenobetaine (cation) (a) C5H12AsO2+ 0.6866 ± 0.0034

Table 2: Reference value for impurities in ABET-1

Substance Molecular formula Mass fraction,
ethanol C2H6O 0.0012 ± 0.0012


The coding refers to the instrumental method of analyte determination.

  • a - Proton nuclear magnetic resonance spectroscopy (1H-qNMR)

Intended use

This reference material is primarily intended for use in the calibration of procedures and the development of methods for the determination of arsenobetaine. A minimum sample mass of 20 mg is recommended.


Included in the combined uncertainty estimate (uc) are uncertainties in the batch characterization (uchar), uncertainties related to possible between-bottle variation (uhom) and uncertainties related to inconsistency between the various measurement methods (umethod). Expressed as standard uncertainties, these components are listed in Table 3 [3].

Table 3: Uncertainty components for ABET-1

Substance uc,
arsenobetaine bromide 0.0025 0.0012 0.0020 0.0009
arsenobetaine (cation) 0.0017 0.0009 0.0014 0.0006
ethanol 0.0006 0.0000 0.0004 0.0004

Date of issue: March 2016
Date of expiry: March 2026

Date modified: