DNA sequencing, gene synthesis and molecular biology services

By applying the technologies listed below, NRC can provide low- and high-throughput sequencing and synthesis, transcriptomics, colony picking, plasmid preparation and gene libraries for companies and organizations requiring technical services in line with genomics and biotechnology projects.

  • Three ABI 3730xl capillary electrophoresis DNA analyzers focused on genomic projects and collaborations where a 96 and 384-well format is used for high-throughput sequencing and genotyping.
  • Pharmacia Phastsystem, Agilent Bioanalyzer and Bio-Rad CHEF Mapper electrophoresis equipment for rapid analysis of proteins and nucleic acids or separation of large DNA molecules.
  • Roche 454 Genome Sequencer FLX instrument with Titanium chemistry capable of generating over 1 million 400bp reads or >500Mbp of sequence data per run and supports many applications including de novo DNA sequencing of whole genomes or large insert clones (BACs), transcriptome profiling, SNP discovery and metagenomics.
  • Illumina Genome Analyzer IIx platform that can produce >150 million 2x75bp reads, or up to 50Gbp of data per run that enable genome sequencing and re-sequencing as well as high depth coverage of samples for applications such as deep transcriptome profiling and small RNA analysis at a relatively low cost.
  • Bioautomation MerMade Model 192 DNA Synthesizer for custom synthesis of DNA molecules up to 150 bases long. The unit provides custom gene synthesis, gene libraries and codon bias adjustments as well as modified DNA including phosphorylated, biotinylated or fluorescent labeled oligonucleotides. Many other custom modifications are available on request.
  • Two Dianex High Performance Liquid Chromatography (HPLC) systems enable custom purification of oligonucleotides and PCR products.
  • Combimatrix B3 microarray synthesizer capable of making custom 94K DNA microarrays.
  • Roche Lightcycler Quantitative PCR for gene expression analysis
  • QPIX colony picker for picking and arraying bacterial colonies
  • Facilities for all standard molecular biology methods including DNA & RNA extraction and quantitation, PCR, gel electrophoresis, cloning, etc.

For information about DNA sequencing, gene synthesis and molecular biology services:

Contact

Paul Neima
Telephone: 902-566-7444
Email: Paul.Neima@nrc-cnrc.gc.ca