Applied genomics

Technical service highlights

We provide a suite of DNA sequencing services to a broad client base; from small-scale Sanger sequencing to large-scale projects using Next Generation Sequencing (NGS) technologies. We also offer a range of molecular techniques for complex genome sequencing, with a particular specialization in de novo sequencing of plant genomes and the genomes of their pathogens and pests. The sequencing platforms can be used for robust single nucleotide polymorphism (SNP) discovery, transcriptome analysis, and metagenomic analysis. We stay current with instrument platforms and available sequencing technologies. Additionally, we provide synthesized DNA of high quality and purity, along with rapid turnaround times. Our in-house bioinformatics group can assist with your data analysis needs.

What we offer

We host a range of sequencing platforms, which allow you to access various technologies and their associated advantages. By providing cost-effective access to DNA sequencing technologies, we can save clients the capital expense of sequencing platforms and the direct bioinformatics costs associated with NGS sequencing.

For small-scale sequencing, such as a single bacterial colony or a single isolated DNA sample, we employ ABI 3730xl DNA Sequencers, which use the proven long read Sanger technology. This technology can be scaled to 96- or 384-well plate format.

We also provide the latest in NGS sequencing to provide Illumina's high depth coverage on the HiSeq 2500 or MiSeq platforms. This enables us to produce significant amounts of data for de novo sequencing of large genomes and re-sequencing for SNP discovery, as well as high depth coverage of samples for applications such as deep transcriptome profiling and small RNA analysis. In addition, we provide long read NGS with the Roche 454 FLX+ Genome Sequencer. This platform supports applications such as sequencing and assembly of smaller repeat-rich genomes or large insert clones (BACs), metagenomics applications and large insert mate pair sequencing (2 to 40kb) for robust genome assembly in combination with Illumina data.

Our specialized DNA synthesis unit can prepare high-quality long oligonucleotides, up to 150 bases in length, ideally suited for the generation of adapters for NGS library construction. The unit can also support the synthesis of modified DNA including phosphorylated, biotinylated or fluorescent-labeled oligonucleotides.

Why work with us

We can be your reliable sequencing provider. NRC has generated high-quality data and this experience has developed a team with expertise in a wide range of genomics and bioinformatics applications for your large genome sequencing and assembly needs. We can respond to your custom sequencing requests and offer a wide range of sequencing options, working with you to determine the best approach for your DNA sequencing requirements.

Fees

We offer specialized services at competitive rates, which include the support of scientific and technical experts and full range of analytical capabilities. We also offer the opportunity for collaboration and/or for our partners to co-locate with us. Contact us to take advantage of our Canada-wide networking, research and development, as well as business development opportunities.

Contact

Business Related Inquiries

Laurel O’Connor
Telephone: 306-975-4573
Email: Laurel.OConnor@nrc-cnrc.gc.ca

Technical Related Inquiries

Inge Roewer, Technical Officer
Telephone: 306-975-6590
Email: Inge.Roewer@nrc-cnrc.gc.ca

Supplemental content

Contact us

Would you like to know more about our applied genomic services? If so, contact us:

Inge Roewer, Technical Officer
Telephone: 306-975-6590
Email: Inge.Roewer@nrc-cnrc.gc.ca

Targeted industry

Agriculture, academia, biotechnology, government, health

Location

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