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Title: |
Development of a reverse Cumate transactivator (rcTA): application for protein production in CHO cells. Also called: Expression system, components thereof and method of use |
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NRC no.: |
11648 |
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Sector(s): |
Bioprocess |
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Inventors: |
Massie, Bernard; Mullick, Alaka; Xu, Yan. |
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Status: |
United States, Application number 60/614,992, Filed 2004-03-10 |
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LOD |
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Abstract: |
Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides. |
For more information, please contact:
Christine St-Jean
Intellectual Property Coordinator, Industrial Affairs
E-mail: christine.st-jean@cnrc-nrc.gc.ca
Note: Data on abstracts is shown in the official language in which it was submitted.
