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Jean-Marc Baribeau
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Raman Scattering Spectroscopy
The interaction of a monochromatic light with a collection of molecules can result in light being scattered by the species under interrogation. The inelastically scattered light, known as the Raman photon, is characteristic of the molecule and of the chemical bonding. Raman spectra, therefore, provide unique fingerprint signature which enables identification of the molecular structure probed. Raman spectroscopy is a non-destructive analytical technique with applications ranging from pharmaceutical, semiconductor, materials science, life science, chemistry, physics, geology, enviromental sciences to archeological artifacts. In addition to chemical structure and bonding information, Raman technique is also used widely in the study of solid phase materials. In particular, it is widely applied in the semiconductor industry to monitor stress, contamination, defect, doping effect, heterostructures and superlattice structures. Coupling this powerful technique to a confocal microscope propell this technique into a very powerful tool to image biological species such as cells or tissue samples.
The Institute operates a LabRam HR(Horiba Jobin Yvon) Confocal Raman microscope. This instrument has high throuput and high resolution and uses multiple excitation sources; 488 nm, 514 nm, 458 nm, 632 nm and 785 nm. A BX51 Olympus optical microscope is integrated with the Raman spectrometer. It is equipped with several specialized objectives (high numerical aperture, long working distance or immersion objectives). A fully motorized and computer controlled automatic stage enables confocal Raman hyperspectral imaging. It’s depth profiling option further allows the study of any transparent samples to build up a 3D Raman image. The thermoelectric cooled CCD detector and a liquid nitrogen cooled InGaAs detectors covered a winde spectral range from 300 nm to 1700 nm. An fiber input port allows users to bypass the microscope systems and import signals from any remote experiments. A fully integrated FTIR spectrometer enables the infrared absorption spectroscopy and microscopy.
The IMS facility also houses the ultra-high spectral resolution triple spectrograph Raman microscope (Horiba Jobin Yvon T64000). The system is configured to be easily switched between additive and subtractive modes. Operating in the subtractive configuration, very low frequency measurements is achievable. In the additive mode, dispersion length of the spectrometer exceeds 1.8 meter, ensures ultra-high spectral resolution.
The confocal Raman microscope is primarily used in biological applications and in the development of a surface enhanced Raman scattering biosensing platform for pathogen detection. Work on the high resolution Raman spectrometer is focused on structural studies of semiconductors thin films and nanostructures.
