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- NRC Canadian Neutron Beam Centre
- NRC Institute for Biological Sciences
- NRC Institute for Chemical Process and Environmental Technology
- NRC Institute for Fuel Cell Innovation
- NRC Institute for Marine Biosciences
- NRC Institute for National Measurement Standards
- NRC Institute for Nutrisciences and Health
- NRC Steacie Institute for Molecular Sciences
- National Bioproducts Program
- National Institute for Nanotechnology
- National Research Council Canada
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Contact
Janet Munholland
Phone: 902-426-6653
Fax: 902-426-9413
Email: Janet.Munholland@nrc-cnrc.gc.ca
Omics & Imaging Platform at IMB
Molecular Biology
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Facilities for all standard molecular biology methods including DNA & RNA extraction and quantitation, PCR, gel electrophoresis, cloning, etc.
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Specialized electrophoresis equipment (Agilent Bioanalyzer, Pharmacia Phastsystem, Bio-Rad CHEF Mapper) for rapid analysis of proteins and nucleic acids or separation of large DNA molecules.
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QPIX colony picker for picking and arraying bacterial colonies.
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Small scale genotyping.
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Quantitative PCR (Roche Lightcycler) for gene expression analysis
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Experienced, talented and dedicated technical support staff.
We offer:
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Library (cDNA or genomic) construction and screening.
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Gene isolation and cloning.
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Gene expression analysis by qPCR.
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Integration with microarray, microscopy and Research Informatics platforms.
Research Informatics
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High-speed server with 72 Gb available memory
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Large capacity Sun Enterprise 20000
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18 node cluster used primarily for BLAST searches
We offer:
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A wide variety of informatics software for biology and chemistry.
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Custom scripts to build software pipelines.
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Integrated database and website construction.
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Experience in genomic sequence assembly and analysis.
Microarray
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Axon Genepix 4200A Non-confocal scanner equipped with 4 internal lasers (488, 532, 594, 635nm wavelength) for greater functionality, 5 – 100 micron resolution.
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Industry-leading low-noise digitization technology with 16 bit digital resolution.
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Genepix Pro Software integrates seamlessly with scanner and downstream software.
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Acuity SQL database analysis software offering statistical, normalization, clustering and visualization capabilities.
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Dedicated technical support
We offer:
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Gene Expression Analysis (Hybridization through to scanning)
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Scanned image analysis (Post processing analysis)
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Comparative Genetic Hybridizations (CGH) and miRNA Hybridizations
Imaging
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Scanning Electron Microscope (SEM) equipped with variable-pressure chamber providing high image quality at low voltages (resolution 2560 x 1920 pixels)
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Transmission Electron Microscopy (TEM) equipped with cryostage providing high-contrast imaging (resolution under 0.4nm) on unstained and freeze-fixed materials
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Light and Fluorescence microscopes equipped with differential interference contrast, epi-fluorescence and a high-resolution camera (image outputs 1280 x 1024 pixels) capable of capturing fleeting and low intensity images
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Experienced, dedicated technical support staff
We offer:
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Scanning imaging of firm and soft substances
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Enhanced elemental X-ray microanalysis
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In-situ hybridization to detect gene activity at subcellular levels
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Sophisticated image analysis, including stereology, for improved determination and interpretation of biological processes within cells and tissues
